Mesoporous magnesium silicate-incorporated poly(ε-caprolactone)-poly(ethylene glycol)- poly(ε-caprolactone) bioactive composite beneficial to osteoblast behaviors

Abstract

Yunfei Niu,1,* Wei Dong,1,* Han Guo,2 Yuhu Deng,3 Lieping Guo,1 Xiaofei An,1 Dawei He,1 Jie Wei,3 Ming Li11Department of Orthopedic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China; 2Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, People's Republic of China; 3Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China *These authors contributed equally to this workAbstract: Mesoporous magnesium silicate (m-MS) and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL) composite (m-MPC) was synthesized by solvent casting method. The results suggest that the mechanical properties of compressive strength and elastic modulus, as well as hydrophilicity, of the m-MPC increased with increase of m-MS content in the composites. In addition, the weight loss of the m-MPC improved significantly with the increase of m-MS content during composite soaking in phosphate-buffered saline for 10 weeks, indicating that incorporation of m-MS into PCL-PEG-PCL could enhance the degradability of the m-MPC. Moreover, the m-MPC with 40 w% m-MS could induce a dense and continuous apatite layer on its surface after soaking in simulated body fluid for 5 days, which was better than m-MPC 20 w% m-MS, exhibiting excellent in vitro bioactivity. In cell cultural experiments, the results showed that the attachment and viability ratio of MG63 cells on m-MPC increased significantly with the increase of m-MS content, showing that the addition of m-MS into PCL-PEG-PCL could promote cell attachment and proliferation. The results suggest that the incorporation of m-MS into PCL-PEG-PCL could produce bioactive composites with improved hydrophilicity, degradability, bioactivity, and cytocompatibility.Keywords: PCL-PEG-PCL, degradation, cytocompatibility, cell attachment, cell proliferation