Nature Communications (Jul 2019)

A comprehensive examination of Nanopore native RNA sequencing for characterization of complex transcriptomes

  • Charlotte Soneson,
  • Yao Yao,
  • Anna Bratus-Neuenschwander,
  • Andrea Patrignani,
  • Mark D. Robinson,
  • Shobbir Hussain

DOI
https://doi.org/10.1038/s41467-019-11272-z
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 14

Abstract

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The Oxford Nanopore system is capable of direct RNA sequencing but complex transcriptome analysis still remains to be thoroughly investigated. Here the authors perform sequencing of native RNA as well as cDNA to characterise the strengths and weaknesses of the system.