Insect (black soldier fly larvae) oil as a potential substitute for fish or soy oil in the fish meal-based diet of juvenile rainbow trout (Oncorhynchus mykiss)

Femi J. Fawole; Shyam N. Labh; Md Sakhawat Hossain; Ken Overturf; Brian C. Small; Thomas L. Welker; Ronald W. Hardy; Vikas Kumar

Animal Nutrition (Dec 2021)

Insect (black soldier fly larvae) oil as a potential substitute for fish or soy oil in the fish meal-based diet of juvenile rainbow trout (Oncorhynchus mykiss)

Femi J. Fawole,
Shyam N. Labh,
Md Sakhawat Hossain,
Ken Overturf,
Brian C. Small,
Thomas L. Welker,
Ronald W. Hardy,
Vikas Kumar

Affiliations

Femi J. Fawole: Aquaculture Research Institute, Department of Animal, Veterinary and Food Sciences, University of Idaho, Moscow, ID, 83844, USA; Hagerman Fish Culture Experiment Station, Aquaculture Research Institute, University of Idaho, Hagerman, ID, USA; Department of Aquaculture and Fisheries, University of Ilorin, Ilorin, Nigeria
Shyam N. Labh: Aquaculture Research Institute, Department of Animal, Veterinary and Food Sciences, University of Idaho, Moscow, ID, 83844, USA; Hagerman Fish Culture Experiment Station, Aquaculture Research Institute, University of Idaho, Hagerman, ID, USA
Md Sakhawat Hossain: Hagerman Fish Culture Experiment Station, Aquaculture Research Institute, University of Idaho, Hagerman, ID, USA; Department of Aquaculture, Faculty of Fisheries, Sylhet Agricultural University, Sylhet, 3100, Bangladesh
Ken Overturf: United States Department of Agriculture, Agricultural Research Service, Hagerman Fish Culture Experiment Station, Hagerman, ID, USA
Brian C. Small: Hagerman Fish Culture Experiment Station, Aquaculture Research Institute, University of Idaho, Hagerman, ID, USA
Thomas L. Welker: United States Department of Agriculture, Agricultural Research Service, Hagerman Fish Culture Experiment Station, Hagerman, ID, USA
Ronald W. Hardy: Aquaculture Research Institute, Department of Animal, Veterinary and Food Sciences, University of Idaho, Moscow, ID, 83844, USA
Vikas Kumar: Aquaculture Research Institute, Department of Animal, Veterinary and Food Sciences, University of Idaho, Moscow, ID, 83844, USA; Hagerman Fish Culture Experiment Station, Aquaculture Research Institute, University of Idaho, Hagerman, ID, USA; Corresponding author.

Journal volume & issue: Vol. 7, no. 4
pp. 1360 – 1370

Abstract

Read online

Alternative sources of fish oil (FO) are one of the major problems in aquaculture; therefore, the goal of the present study was to examine insect (black soldier fly larvae) oil (BSLO) as a potential replacer of fish/soy oil in juvenile rainbow trout (initial average weight of 32 ± 0.15 g) feed. Four diets were formulated wherein FO (control diet) was completely replaced with either soybean oil (SO) or BSLO, and an additional BSLO-based diet supplemented with 1.5% bile acid (BSLO + BA) were fed to the fish for 10 weeks. Growth performance of the BSLO fed group was similar (P > 0.05) to that of the FO and SO fed groups, however, the fish fed BSLO + BA diet registered the lowest growth (P 0.05) affect the major nutrient content of whole-body, however, the fatty acid composition of the muscle and liver was influenced (P < 0.05), with the highest 14:0, 16:0, and total saturated fatty acid detected in BSLO or BSLO + BA fed trout compared to the others (P < 0.001). No significant differences were observed in eicosapentaenoic acid + docosahexaenoic acid (EPA + DHA) or total n-3 polyunsaturated fatty acid (PUFA) content in muscle among the groups, whereas, the highest EPA:DHA and n-3:n-6 ratios were detected in the FO group. Gene expression for fatty acid binding protein (fabp), fatty acid synthase (fas), and Δ5 desaturase in the liver was lower in FO (P < 0.05), while BSLO + BA registered the highest Δ6 expression (P = 0.006). Supplementation of BA in the BSLO diet increased superoxide dismutase (SOD) and catalase (CAT) activities compared to the other groups (P < 0.05). In conclusion, BSLO could serve as a substitute for FO and SO in rainbow trout diet without negatively impacting growth performance, whole-body composition and nutrient retention, and modulate the expression of fatty acid metabolism-related genes in rainbow trout.

Published in Animal Nutrition

ISSN: 2405-6545 (Print)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Animal culture
Website: https://www.keaipublishing.com/en/journals/animal-nutrition/

About the journal

Abstract

Keywords