TRANSFORMATION EFFECTIVENESS FOR ARABIDOPSIS THALIANA PLANTS BY DNA-CONSTRUCTIONS WITH SITE-SPECIFIC RECOMBINASE SYSTEM Cre/loxP

Abstract

Using of new approach with site-specific recombinase system Cre/loxP under the control of 35S-promoter to generate marker-free genetically modified plants was developed. The analysis of recombinase system was carried out during the next generation of Arabidopsis thaliana plants, produced by agrobacterium transformation method. For this purpose two types of DNA-constructions were used for establishing better variant. The histochemical analysis of the plants progeny T1 transformed by both construct types was described. As a result of our work, it was established that the amount of marker-free transformants was arising during every next transformation offspring independently of the used construct type. The new strategy provides a simple and rapid way to eliminate swelective and marker genes.

Keywords

• site-specific recombinase system Cre/loxP
• marker genes excision