The effects of abiotic stresses on the NADP-dependent malic enzyme in the leaves of the hexaploid wheat

Abstract

The objective of this study was to examine the effects of different abiotic stresses on the activity of an NADP-dependent malic enzyme (NADP-ME) and the corresponding gene transcription in the leaves of the hexaploid wheat (Triticum aestivum L.) The activity of the NADP-ME enzyme was increased by water stress (20 % polyethylene glycol 6000), low temperature (4 °C), darkness, salinity (200 mM NaCl), abscisic acid and salicylic acid. The transcription of the TaNADP-ME1 gene decreased in response to all of the stresses except darkness and NaCl. In addition, the transcription of TaNADP-ME2 was down-regulated by all of the tested treatments and could not be detected under dark stress.

Keywords

• abscisic acid
• enzyme activity
• gene transcription
• low temperature
• salicylic acid
• salinity
• water stress