Anti-inflammatory Effect of Benincasae Semen Herbal-acupuncture at KI10 on nephritis in rats

Abstract

Objective : This study aimed to evaluate the effects of Benincasae Semen Herbal-acupuncture (BSHA) at KI10 (Umgok) on nephritis induced by LPS in rat. Methods : Rats with nephritis induced by LPS, were treated with Benincasae Semen Herbal-acupuncture(BS-HA) injection at KI10. Two control groups, N.P. group and saline group, were treated with 26gauge needle at KI10, 3 times a week. In Saline group normal saline was injected at KI10. To evaluate the effects of Benincasae Semen Herbal-acupuncture at KI10 on nephritis in rats, WBC, Neutrophils in blood, BUN, Creatinine TNF-α, CINC-1 in serum, urinal volume and creatinine and Total protein in urine, reanl TNF-α, renal MPO were measured and reanl tissue was also analyzed. Results : BS-HA injected at KI10 significantly inhibited WBC and neutrophil in blood, creatinine in serum, and MPO in kidney of LPS-stimulated rats. BS-HA injected at KI10 reduced concentration of neutrophil in renal tissue of LPS-stimulated rats. Conclusion : BS-HA at KI10 has a therapeutic effect for nephritis in LPS-stimulated rat. Therefore, it is suggested that BS-HA at KI10 may be an useful therapeutics in clinical field after further researches.

Keywords

• Nephritis
• Herbalacupuncture
• KI10
• Benincasae Semen
• LPS
• Benincasae Semen