Cholestan Steroids from The Stem Bark of Aglaia angustifolia Miq and Their Cytotoxic Activity against MCF-7 Breast Cancer Cell Lines
Ricson Pemimpin Hutagaol,
Tjandrawati Mozef,
Sofa Fajriah,
Gian Primahana,
Unang Supratman,
Desi Harneti,
Ace Tatang Hidayat,
Khalijah Awang,
Yoshihito Shiono
Affiliations
Ricson Pemimpin Hutagaol
Department of Chemistry, Faculty of Mathematics and Natural sciences, Nusa Bangsa University, Bogor 16166, Indonesia
Tjandrawati Mozef
Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Serpong Tangerang Selatan 15314
Sofa Fajriah
Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Serpong Tangerang Selatan 15314
Gian Primahana
Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Serpong Tangerang Selatan 15314, Indonesia
Unang Supratman
Department of Chemistry, Faculty of Mathematics and Natural sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia
Desi Harneti
Department of Chemistry, Faculty of Mathematics and Natural sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia
Ace Tatang Hidayat
Khalijah Awang
Yoshihito Shiono
Department of Food, Life, and Environmental Science, Faculty of Agriculture, Yamagata University, Tsuruoka, Yamagata 997-8555, Japan
With about 120 species, Aglaia is one of the largest genera of the plant family Meliaceae (the mahogany plants). Various Aglaia species have been investigated since the 1960s for their phytochemical constituents and biological properties. This research objective was to find secondary metabolites that have activity as anti-breast cancer compounds from endemic Indonesian Aglaia, such as Aglaia angustifolia Miq. Two cholestan type steroids, stigmast-5en-3α-acetat (1), as a new steroid with α-sterochemistry of acetyl moiety at C-3 and 23a-homostigmast-5en-3β-ol (2), with unusual side chain were isolated for the first time from the stem bark of Aglaia angustifolia Miq or known as segara tree in Kalimantan. The chemical structures of two steroids were identified with spectroscopic data, including IR, NMR (1H, 13C, DEPT 135°, HMQC, HMBC, NOESY, 1H-1H COSY) and HRTOF-MS, as well as by comparing with previously reported spectral data. These two steroids were isolated for the first time from this genus. Steroids 1 and 2 were evaluated for cytotoxic activity against MCF-7 breast cancer cells and showed weak activity with IC50 values of 829.0 and 903.0 µg/mL, respectively.
