Label-Free Sensing of Biomolecular Adsorption and Desorption Dynamics by Interfacial Second Harmonic Generation

Chuansheng Xia; Jianli Sun; Qiong Wang; Jinping Chen; Tianjie Wang; Wenxiong Xu; He Zhang; Yuanyuan Li; Jianhua Chang; Zengliang Shi; Chunxiang Xu; Qiannan Cui

doi:10.3390/bios12111048

Biosensors (Nov 2022)

Label-Free Sensing of Biomolecular Adsorption and Desorption Dynamics by Interfacial Second Harmonic Generation

Chuansheng Xia,
Jianli Sun,
Qiong Wang,
Jinping Chen,
Tianjie Wang,
Wenxiong Xu,
He Zhang,
Yuanyuan Li,
Jianhua Chang,
Zengliang Shi,
Chunxiang Xu,
Qiannan Cui

Affiliations

Chuansheng Xia: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
Jianli Sun: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
Qiong Wang: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
Jinping Chen: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
Tianjie Wang: School of Electronic and Information Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China
Wenxiong Xu: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
He Zhang: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
Yuanyuan Li: School of Electronic and Information Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China
Jianhua Chang: School of Electronic and Information Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China
Zengliang Shi: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
Chunxiang Xu: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
Qiannan Cui: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China

DOI: https://doi.org/10.3390/bios12111048
Journal volume & issue: Vol. 12, no. 11
p. 1048

Abstract

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Observing interfacial molecular adsorption and desorption dynamics in a label-free manner is fundamentally important for understanding spatiotemporal transports of matter and energy across interfaces. Here, we report a label-free real-time sensing technique utilizing strong optical second harmonic generation of monolayer 2D semiconductors. BSA molecule adsorption and desorption dynamics on the surface of monolayer MoS2 in liquid environments have been all-optically observed through time-resolved second harmonic generation (SHG) measurements. The proposed SHG detection scheme is not only interface specific but also expected to be widely applicable, which, in principle, undertakes a nanometer-scale spatial resolution across interfaces.

Published in Biosensors

ISSN: 2079-6374 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.mdpi.com/journal/biosensors

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