Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats

Guoying Zhu; Li Chen; Su Liu; Ling She; Yongnian Ding; Changqing Yang; Fengshang Zhu

Heliyon (Jul 2022)

Celecoxib-mediated attenuation of non-alcoholic steatohepatitis is potentially relevant to redistributing the expression of adiponectin receptors in rats

Guoying Zhu,
Li Chen,
Su Liu,
Ling She,
Yongnian Ding,
Changqing Yang,
Fengshang Zhu

Affiliations

Guoying Zhu: Department of Clinical Nutrition, Putuo People's Hospital, School of Medicine, Tongji University, Shanghai, 200060, China
Li Chen: Department of Gastroenterology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, 200065, China; State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia & Xinjiang Key Laboratory of Neurological Disorder Research, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830000, China; Department of Gastroenterology, Second Affiliated Hospital of Xinjiang Medical University, Urumqi, 830063, China
Su Liu: Department of Gastroenterology, Zhabei Central Hospital of Jingan District, Shanghai, 200070, China
Ling She: State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia & Xinjiang Key Laboratory of Neurological Disorder Research, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830000, China; Department of Gastroenterology, Second Affiliated Hospital of Xinjiang Medical University, Urumqi, 830063, China
Yongnian Ding: State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia & Xinjiang Key Laboratory of Neurological Disorder Research, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830000, China; Department of Gastroenterology, Second Affiliated Hospital of Xinjiang Medical University, Urumqi, 830063, China
Changqing Yang: Department of Gastroenterology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, 200065, China
Fengshang Zhu: State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia & Xinjiang Key Laboratory of Neurological Disorder Research, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830000, China; Department of Gastroenterology, Second Affiliated Hospital of Xinjiang Medical University, Urumqi, 830063, China; Department of Gastroenterology, Tongji Hospital, School of Medicine, Tongji University, 389 Xincun Road, Shanghai, 200065, China; Corresponding author.

Journal volume & issue: Vol. 8, no. 7
p. e09872

Abstract

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Pharmacological inhibition of cyclooxygenase-2 (COX-2) activity ameliorated the severity of non-alcoholic steatohepatitis (NASH) rats. It is not completely understood that the role of COX-2 inhibitor celecoxib on adiponectin receptors (Adipo-R1/R2) expression in different tissues in NASH rats. Sprague-Dawley male NASH rats induced by a high-fat diet (HFD) were administrated with or without celecoxib for 8 weeks. Biochemical parameters of liver function, glucose, and lipid metabolism, and the levels of adiponectin, tumor necrosis factor-alpha (TNF-α), prostaglandin E2 (PGE2) in the serum or liver were collected according to the standard protocols. The mRNA and protein levels of Adipo-R1, Adipo-R2, and COX-2 in the liver, muscle, and visceral fat were performed by quantitative real-time polymerase chain reaction (q-PCR) and Western blot analysis, respectively. The results showed that celecoxib ameliorated the various clinical indicators and pathological characteristics in the NASH rats, including body weight, liver function, liver index, and redox activities in serum and hepatic samples. The serum concentrations of adiponectin and TNF-α and PGE2 were negatively correlated. As expected, these ameliorative effects of celecoxib were associated with the gene and protein levels up-regulation of Adipo-R1, Adipo-R2 in the liver and visceral fat tissues, and seeming to be compensatory down-regulation expression in muscle tissues (P <0.05). Additionally, COX-2 protein expression was negatively correlated with serum adiponectin levels, protein expression of adiponectin receptors from the liver and visceral fat, conversely, positively correlated with those from the muscle. Our current study demonstrate that celecoxib might effectively alleviate NASH rats in a unique manner closely relevant to redistributing the expression of adiponectin receptors in the liver, visceral fat, and muscle. However, the precise molecular mechanism needs further study.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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