TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells

Rubin Narayan Joshi; Charlotte Stadler; Robert Lehmann; Janne Lehtiö; Jesper Tegnér; Jesper Tegnér; Angelika Schmidt; Mattias Vesterlund

doi:10.3389/fimmu.2019.02708

Frontiers in Immunology (Nov 2019)

TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells

Rubin Narayan Joshi,
Charlotte Stadler,
Robert Lehmann,
Janne Lehtiö,
Jesper Tegnér,
Jesper Tegnér,
Angelika Schmidt,
Mattias Vesterlund

Affiliations

Rubin Narayan Joshi: Unit of Computational Medicine, Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institutet, Karolinska University Hospital and Science for Life Laboratory, Stockholm, Sweden
Charlotte Stadler: Department of Protein Sciences, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH - Royal Institute of Technology, Science for Life Laboratory, Solna, Sweden
Robert Lehmann: Biological and Environmental Sciences and Engineering Division, Computer, Electrical and Mathematical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Janne Lehtiö: Department of Oncology and Pathology, Karolinska Institutet, Science for Life Laboratory, Solna, Sweden
Jesper Tegnér: Unit of Computational Medicine, Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institutet, Karolinska University Hospital and Science for Life Laboratory, Stockholm, Sweden
Jesper Tegnér: Biological and Environmental Sciences and Engineering Division, Computer, Electrical and Mathematical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Angelika Schmidt: Unit of Computational Medicine, Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institutet, Karolinska University Hospital and Science for Life Laboratory, Stockholm, Sweden
Mattias Vesterlund: Department of Oncology and Pathology, Karolinska Institutet, Science for Life Laboratory, Solna, Sweden

DOI: https://doi.org/10.3389/fimmu.2019.02708
Journal volume & issue: Vol. 10

Abstract

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We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 min and 1 h of T cell receptor (TCR) stimulation, respectively. We quantified the subcellular distribution of 6,572 proteins and identified a subset of 237 potentially translocating proteins, including both well-known examples and novel ones. Microscopic validation confirmed the localization of selected proteins with previously known and unknown localization, respectively. We further provide the data in an easy-to-use web platform to facilitate re-use, as the data can be relevant for basic research as well as for clinical exploitation of T cells as therapeutic targets.

Published in Frontiers in Immunology

ISSN: 1664-3224 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: http://journal.frontiersin.org/journal/immunology

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