STRUCTURAL DIVERSITY IN BETA-TUBULIN ISOTYPE 1 PROTEIN OF BENZIMIDAZOLE RESISTANT AND SUSCEPTIBLE HAEMONCHUS CONTORTUS

Abstract

Reports of benzimidazole resistance across the globe are available. The resistance of the parasite to the benzimidazole group is due to the single nucleotide polymorphisms at three positions, 200, 198, and 167 of the β-tubulin isotype 1 gene. The mutation at position 200 is commonly reported in various parts of India. The present study analyzed the genetic diversity in ten isolates of Haemonchus contortus β-tubulin isotype 1 gene in different states of India. In silico models of susceptible and F200Y mutated β-tubulin were built and docking with albendazole was done. The result of the docking study showed the alteration in the conformation of the albendazole binding pocket of F200Y mutated β-tubulin in comparison to the susceptible β-tubulin model.

Keywords

• β-tubulin
• benzimidazole resistance
• haemonchus contortus
• molecular docking
• mutation