The study aimed to evaluate the agronomic performance and the physicochemical characteristics of Americana, Crespa and Mimosa organic lettuces. American, Crespa and Mimosa organic lettuce seedlings were treated with Supermagro; seaweed extract; fish hydrolysate and water application. In this study, we observed that the use of biofertilizer, regardless of the type of lettuce, does not interfere with the production and head diameter, affecting only the dry matter content. The pH, soluble solids content and titratable acidity varied according to the treatments, showing interaction. The Americana type obtained higher agronomic performance as compared to others.