Study of the acute toxicity of the medicinal product for veterinary use Iverbutan

Abstract

The purpose of the research is to study the acute oral toxicity of the medicinal product for veterinary use Iverbutan, intended for the treatment and prevention of arachnoentomoses and nematodoses of poultry.Materials and methods. The studies were carried out on 30 outbred male rats weighing 210-240 g and 60 mice weighing 18–21 g. The animals were divided into experimental and control groups. The drug was administered once without dilution in the form of the provided solution using an intragastric tube. Doses of 2000, 4000, 6000, 8000 and 10 000 mg/kg were tested on mice, and on rats – 10 000, 8000, 5000, 4000 mg/kg. The animals of the control groups were injected with drinking water. Within 14 days after a single dose of the drug, the physiological state and behavior of animals, possible death, as well as the manifestation of symptoms of intoxication were monitored. The control of the body weight of the animals of the experimental and control groups was carried out on the day of the experiment (before drug administration), as well as on the 1st, 3rd, 7th, 9th and 14th days.Results and discussion. It was found that after oral administration of iverbutan to experimental animals, the average lethal dose, calculated by the Kerber method, was 5600 mg/kg of body weight in mice and 7000 mg/kg of body weight in rats (hazard class 4 according to GOST 12.1.007-76). The average lethal dose, calculated by the Miller and Tainter method, was 5292.0±1058.6 (4233.4÷6350.6) mg/kg of body weight in mice and 6463.2±1496.9 (4966.3÷7960.1) mg/kg of body weight of rats (hazard class 3 according to GOST 12.1.007-76), which indicates species sensitivity.

Keywords

• iverbutan
• ivermectin
• acute toxicity
• mice
• rats
• medium lethal dose