Antifungal Properties of Bioactive Compounds Isolated from <i>Fucus vesiculosus</i> Supercritical Carbon Dioxide Extract
Katarzyna Tyśkiewicz,
Felix Rüttler,
Renata Tyśkiewicz,
Artur Nowak,
Marcin Gruba,
Anita Wziątek,
Agnieszka Dębczak,
Michał Sandomierski,
Walter Vetter
Affiliations
Katarzyna Tyśkiewicz
Department of Food Chemistry (170B), Institute of Food Chemistry, University of Hohenheim, Garbenstraβe 28, D-70599 Stuttgart, Germany
Felix Rüttler
Department of Food Chemistry (170B), Institute of Food Chemistry, University of Hohenheim, Garbenstraβe 28, D-70599 Stuttgart, Germany
Renata Tyśkiewicz
Analytical Laboratory, Łukasiewicz Research Network—New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland
Artur Nowak
Department of Industrial and Environmental Microbiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie–Skłodowska University, Akademicka 19, 20-033 Lublin, Poland
Marcin Gruba
Supercritical Extraction Research Group, Łukasiewicz Research Network—New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland
Anita Wziątek
Supercritical Extraction Research Group, Łukasiewicz Research Network—New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland
Agnieszka Dębczak
Supercritical Extraction Research Group, Łukasiewicz Research Network—New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland
Michał Sandomierski
Supercritical Extraction Research Group, Łukasiewicz Research Network—New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland
Walter Vetter
Department of Food Chemistry (170B), Institute of Food Chemistry, University of Hohenheim, Garbenstraβe 28, D-70599 Stuttgart, Germany
The exploration of natural antifungal substances from algal origins is significant due to the increasing resistance of pathogens to conventional antifungal agents and the growing consumer demand for natural products. This manuscript represents the inaugural investigation into the antifungal attributes of bioactive compounds extracted from Fucus vesiculosus via supercritical carbon dioxide (scCO2) extraction utilizing contemporary countercurrent chromatography (CCC). In aligning with the prospective utilization of this extract within the agricultural sector, this study also serves as the preliminary report demonstrating the capability of Fucus vesiculosus scCO2 extract to enhance the activity of plant resistance enzymes. The fractions obtained through CCC were subjected to evaluation for their efficacy in inhibiting the macrospores of Fusarium culmorum. The CCC methodology facilitated the successful separation of fatty acids (reaching up to 82.0 wt.% in a given fraction) and fucosterol (attaining up to 79.4 wt.% in another fraction). All CCC fractions at the concentration of 1.0% were found to inhibit 100% of Fusarium culmorum growth. Moreover, Fucus vesiculosus scCO2 extract was able to activate plant resistance enzymes (Catalase, Ascorbic Peroxidase, Guaiacol Peroxidase, Phenylalanine Ammonia-Lyase, and Phenylalanine Ammonia-Lyase Activity).
