Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe

Abstract

Summary: Current fire blight diagnosis techniques are DNA based and require specialized equipment and expertise, or they are less sensitive. Here, we present a protocol for diagnosing fire blight using the fluorescent probe, B-1. We describe steps for Erwinia amylovora culture, implementing a fire blight-infected model, and E. amylovora visualization. This protocol allows for detection of fire blight bacteria of up to 102 CFU/mL on plants or objects in just 10 s with a simple application including spraying and swabbing.For complete details on the use and execution of this protocol, please refer to Jung et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Material Sciences
• Model Organisms
• Plant Sciences