Морфологія (Oct 2020)
Submicroscopic changes of the spleen in the action of monosodium glutamate and correction with melatonin
Abstract
Background. Monosodium glutamate is one of the most common dietary supplements in the world. The aim of the study was to study the electron microscopic changes in the structural components of the spleen of rats under the action of monosodium glutamate and its correction with melatonin. Methods. The experimental study was performed on 66 white male rats and females of reproductive age. For six weeks, the animals received monosodium glutamate at a dose of 0.07 g/kg body weight daily with food, after which they switched to a standard diet with the addition of melatonin at a dose of 0.01 g/kg for two, four and six weeks. Sections of the spleen were made on an ultramicrotome UMTP-6M with a diamond knife (DIATOM) and double contrast was performed according to Reynolds and uranyl acetate. Submicroscopic examinations of the organ were performed using an electron transmission microscope TEM-100. The test material was documented using a SONY – H9 digital camera. Results. Daily consumption of monosodium glutamate for six weeks leads to profound destructive changes in the cellular composition of the spleen, causing disorders in the vascular bed. In particular, the number of lymphocytes with signs of apoptosis increases significantly, and the number of cells with signs of mitosis decreases, in the cytoplasm of dendritic cells organelles with signs of damage, in the periarterial zone signs of edema, a large number of active macrophages, the cytoplasm of which contains osmophilic inclusions and remnants of phagocytosed cells. The red pulp is full-blooded, contains areas of accumulation of deformed blood cells, polysegmental neutrophils and megakaryocytes. Blood capillaries with reduced lumen. The introduction of melatonin leads to a significant restoration of the structural organization and, consequently, the function of this organ. Conclusion. Melatonin has a positive effect on the restoration of changes in the spleen caused by the action of monosodium glutamate.
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