مجله علوم پزشکی فیض (پیوسته) (Jun 2024)
Comparison of the effects of eight weeks of combined endurance-resistance training and taurine supplementation on the expression of cardiac AKT and PI3K genes and serum IL-1β levels in diabetic male rats
Abstract
Background and Aim: AKT, PI3K, and IL-1β are associated with diabetes and cardiac inflammation. This study aimed to compare the interactive effects of eight weeks of combined endurance-resistance training and taurine supplementation on the expression of the AKT gene, cardiac PI3K, and serum IL-1β levels in diabetic male Wistar rats. Methods: In this experimental study, 40 adult male Wistar rats, weighing between 250-300 grams and aged six weeks, were randomly divided into four groups: diabetic control, diabetic with combined training, diabetic with supplementation, and a non-diabetic healthy control group (10 rats in each group). Diabetes was induced by administering 55 mg/kg body weight of streptozocin via intraperitoneal injection. Blood glucose levels above 250 mg/dL were considered indicative of diabetes. Combined resistance-endurance exercises were performed five times a week for eight weeks. Taurine supplementation was provided as a 1% solution in water daily for the same duration. Serum IL-1β levels were measured using ELISA, and the expression of AKT and PI3K genes was assessed via Western blot analysis. Results: The expression of the PI3K gene (P=0.002) and serum IL-1β levels (P=0.001) significantly increased in the diabetic combined training group compared to the diabetic control group. However, there were no significant changes observed in the diabetic supplementation group compared to the diabetic control group. Additionally, the expression of the AKT gene did not show significant changes in either the diabetic combined training or diabetic supplementation groups compared to the diabetic control group. ANOVA results indicated that in the diabetic combined training group, the level of PI3K gene expression increased by 57%, while serum IL-1β levels increased by 42% compared to the diabetic control group. Conclusion: Combined endurance-resistance training appears to enhance the expression of PI3K and IL-1β, leading to favorable changes in glucose metabolism and improved cardiac inflammatory markers in diabetic rats compared to taurine supplementation alone.
