Temporal oral microbiome changes with brushing in children with cleft lip and palate
Rita Rodrigues,
Ana P. Chung,
Martin S. Mortensen,
Maria H. Fernandes,
António B. Monteiro,
Rowney Furfuro,
Cátia C. Silva,
Maria C. Manso,
Søren J. Sørensen,
Paula V. Morais
Affiliations
Rita Rodrigues
Faculty of Dentistry, University of Porto, R. Dr. Manuel Pereira da Silva, 93, 4200-393, Porto, Portugal; Faculty of Health Sciences, University Fernando Pessoa, Praça 9 de Abril, 349, 4249-004, Porto, Portugal
Ana P. Chung
University of Coimbra, Centre for Mechanical Engineering, Materials and Processes, Department of Life Sciences, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal
Martin S. Mortensen
Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200, Copenhagen N, Denmark
Maria H. Fernandes
Associated Laboratory for Green Chemistry (LAQV/REQUIMTE), University of Porto, 4051-401, Porto, Portugal
António B. Monteiro
Lusiadas Hospital, Av. da Boavista 171, 4050-113, Porto, Portugal
Rowney Furfuro
Compor Clinic, R. Aval de Cima 34, 4200-105, Porto, Portugal
Cátia C. Silva
Faculty of Health Sciences, University Fernando Pessoa, Praça 9 de Abril, 349, 4249-004, Porto, Portugal
Maria C. Manso
Associated Laboratory for Green Chemistry (LAQV/REQUIMTE), University of Porto, 4051-401, Porto, Portugal; Faculty of Health Sciences, University Fernando Pessoa, Fernando Pessoa Energy, Environment and Health Research Unit (FP-ENAS), Praça de 9 de Abril, 349, 4249-004, Porto, Portugal
Søren J. Sørensen
Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200, Copenhagen N, Denmark
Paula V. Morais
University of Coimbra, Centre for Mechanical Engineering, Materials and Processes, Department of Life Sciences, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal; Corresponding author.
This cohort study aimed to characterize the oral microbiome of children with CLP, from two different age groups, and evaluate the effect of supervised or unsupervised toothbrushing on the microbiome of the cleft over time. Swab samples were collected from the cleft area at three different time points (A; no brushing, B; after 15 days and C; after 30 days) and were analyzed using next-generation sequencing to determine the microbial composition and diversity in these time points. Overall, brushing significantly decreased the abundance of the genera Alloprevotella and Leptotrichia in the two age groups examined, and for Alloprevotella this decrease was more evident for children (2–6 years old). In the preteen group (7–12 years old), a significant relative increase of the genus Rothia was observed after brushing. In this study, the systematic brushing over a period of thirty days also resulted in differences at the intra-individual bacterial richness.
