Cysteamine Supplementation In Vitro Remarkably Promoted Rumen Fermentation Efficiency towards Propionate Production via <i>Prevotella</i> Enrichment and Enhancing Antioxidant Capacity
Qichao Wu,
Hewei Chen,
Fan Zhang,
Weikang Wang,
Fengliang Xiong,
Yingyi Liu,
Liangkang Lv,
Wenjuan Li,
Yukun Bo,
Hongjian Yang
Affiliations
Qichao Wu
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agri-Cultural University, Beijing 100193, China
Hewei Chen
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agri-Cultural University, Beijing 100193, China
Fan Zhang
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agri-Cultural University, Beijing 100193, China
Weikang Wang
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agri-Cultural University, Beijing 100193, China
Fengliang Xiong
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agri-Cultural University, Beijing 100193, China
Yingyi Liu
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agri-Cultural University, Beijing 100193, China
Liangkang Lv
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agri-Cultural University, Beijing 100193, China
Wenjuan Li
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agri-Cultural University, Beijing 100193, China
Yukun Bo
Animal Husbandry Technology Promotion Institution of Zhangjiakou, Zhangjiakou 075000, China
Hongjian Yang
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agri-Cultural University, Beijing 100193, China
Cysteamine (CS) is a vital antioxidant product and nutritional regulator that improves the productive performance of animals. A 2 × 4 factorial in vitro experiment was performed to determine the effect of the CS supplementation levels of 0, 20, 40, and 60 mg/g, based on substrate weight, on the ruminal fermentation, antioxidant capacity, and microorganisms of a high-forage substrate (HF, forage:corn meal = 7:3) in the Statistical Analysis System Institute. After 48 h of incubation, the in vitro dry matter disappearance and gas production in the LF group were higher when compared with a low-forage substrate (LF, forge hay:corn meal = 3:7), which was analyzed via the use of the MIXED procedure of the HF group, and these increased linearly with the increasing CS supplementation (p p p p p p Bacteroidota, instead of Firmicutes, was in the LF group than in the HF group. Furthermore, increasing the CS supplementation linearly increased the relative abundance of Prevotella, norank_f_F082, and Prevotellaceae_UCG-001 under the two substrates (p Prevotella, norank_f_F082, and Prevotellaceae_UCG-001 were positively correlated with gas production, rumen fermentation, and antioxidant capacity in a Spearman correlation analysis (r > 0.31, p Prevotella in Bacteroidetes.
