Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples

Mauro Tomassetti; Giovanni Merola; Elisabetta Martini; Luigi Campanella; Gabriella Sanzò; Gabriele Favero; Franco Mazzei

doi:10.3390/s17040819

Sensors (Apr 2017)

Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples

Mauro Tomassetti,
Giovanni Merola,
Elisabetta Martini,
Luigi Campanella,
Gabriella Sanzò,
Gabriele Favero,
Franco Mazzei

Affiliations

Mauro Tomassetti: Department of Chemistry, “Sapienza” University of Rome, P.le A. Moro, 5, 00185 Rome, Italy
Giovanni Merola: Department of Chemistry, “Sapienza” University of Rome, P.le A. Moro, 5, 00185 Rome, Italy
Elisabetta Martini: Department of Chemistry, “Sapienza” University of Rome, P.le A. Moro, 5, 00185 Rome, Italy
Luigi Campanella: Department of Chemistry, “Sapienza” University of Rome, P.le A. Moro, 5, 00185 Rome, Italy
Gabriella Sanzò: Department of Chemistry and Pharmacy Technology, “Sapienza” University of Rome, P.le A. Moro, 5, 00185 Rome, Italy
Gabriele Favero: Department of Chemistry and Pharmacy Technology, “Sapienza” University of Rome, P.le A. Moro, 5, 00185 Rome, Italy
Franco Mazzei: Department of Chemistry and Pharmacy Technology, “Sapienza” University of Rome, P.le A. Moro, 5, 00185 Rome, Italy

DOI: https://doi.org/10.3390/s17040819
Journal volume & issue: Vol. 17, no. 4
p. 819

Abstract

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In this research, we developed a direct-flow surface plasmon resonance (SPR) immunosensor for ampicillin to perform direct, simple, and fast measurements of this important antibiotic. In order to better evaluate the performance, it was compared with a conventional amperometric immunosensor, working with a competitive format with the aim of finding out experimental real advantages and disadvantages of two respective methods. Results showed that certain analytical features of the new SPR immunodevice, such as the lower limit of detection (LOD) value and the width of the linear range, are poorer than those of a conventional amperometric immunosensor, which adversely affects the application to samples such as natural waters. On the other hand, the SPR immunosensor was more selective to ampicillin, and measurements were more easily and quickly attained compared to those performed with the conventional competitive immunosensor.

Published in Sensors

ISSN: 1424-8220 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/sensors

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