circ-Amotl1 in extracellular vesicles derived from ADSCs improves wound healing by upregulating SPARC translation

Dazhou Wu; Shengyi Chen; Dongdong Huang; Zhipeng Huang; Na Zhen; Zhenxu Zhou; Jicai Chen

Regenerative Therapy (Mar 2024)

circ-Amotl1 in extracellular vesicles derived from ADSCs improves wound healing by upregulating SPARC translation

Dazhou Wu,
Shengyi Chen,
Dongdong Huang,
Zhipeng Huang,
Na Zhen,
Zhenxu Zhou,
Jicai Chen

Affiliations

Dazhou Wu: Department of Hernia and Abdominal Wall Surgery, The First Affiliated Hospital of Wenzhou Medical University, PR China
Shengyi Chen: Department of Hernia and Abdominal Wall Surgery, The First Affiliated Hospital of Wenzhou Medical University, PR China
Dongdong Huang: Department of Hernia and Abdominal Wall Surgery, The First Affiliated Hospital of Wenzhou Medical University, PR China
Zhipeng Huang: Department of Hernia and Abdominal Wall Surgery, The First Affiliated Hospital of Wenzhou Medical University, PR China
Na Zhen: Department of Hernia and Abdominal Wall Surgery, The First Affiliated Hospital of Wenzhou Medical University, PR China
Zhenxu Zhou: Department of Hernia and Abdominal Wall Surgery, The First Affiliated Hospital of Wenzhou Medical University, PR China
Jicai Chen: Corresponding author. Department of Hernia and Abdominal Wall Surgery, The First Affiliated Hospital of Wenzhou Medical University, Shangcai County, South Baixiang Street, Ouhai District, Wenzhou city, Zhejiang Province, 325000, PR China.; Department of Hernia and Abdominal Wall Surgery, The First Affiliated Hospital of Wenzhou Medical University, PR China

Journal volume & issue: Vol. 25
pp. 290 – 301

Abstract

Read online

Aim: This study aims to explore the mechanism of circ- AMOT-like protein 1 (Amotl1) in extracellular vesicles (Evs) derived from adipose-derived stromal cells (ADSCs) regulating SPARC translation in wound healing process. Methods: The morphology, wound healing rate of the wounds and Ki67 positive rate in mouse wound healing models were assessed by H&E staining and immunohistochemistry (IHC). The binding of IGF2BP2 and SPARC was verified by RNA pull-down. Adipose-derived stromal cells (ADSCs) were isolated and verified. The Evs from ADSCs (ADSC-Evs) were analyzed. Results: Overexpression of SPARC can promote the wound healing process in mouse models. IGF2BP2 can elevate SPARC expression to promote the proliferation and migration of HSFs. circ-Amotl1 in ADSC-Evs can increase SPARC expression by binding IGF2BP2 to promote the proliferation and migration of HSFs. Conclusion: ADSC-Evs derived circ-Amotl1 can bind IGF2BP2 to increase SPARC expression and further promote wound healing process.

Published in Regenerative Therapy

ISSN: 2352-3204 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General): Cytology
Website: http://www.journals.elsevier.com/regenerative-therapy/

About the journal

Abstract

Keywords