Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21

Francesca Cantini; Paola Giannì; Sara Bobone; Cassandra Troiano; Hugo van Ingen; Renato Massoud; Nicoletta Perini; Luciana Migliore; Philippe Savarin; Charles Sanders; Lorenzo Stella; Marco Sette

doi:10.3390/membranes13030365

Membranes (Mar 2023)

Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21

Francesca Cantini,
Paola Giannì,
Sara Bobone,
Cassandra Troiano,
Hugo van Ingen,
Renato Massoud,
Nicoletta Perini,
Luciana Migliore,
Philippe Savarin,
Charles Sanders,
Lorenzo Stella,
Marco Sette

Affiliations

Francesca Cantini: Magnetic Resonance Center (CERM), University of Florence, Sesto Fiorentino, 50019 Firenze, Italy
Paola Giannì: Department of Chemical Sciences and Technology, University of Rome Tor Vergata, 00133 Rome, Italy
Sara Bobone: Department of Chemical Sciences and Technology, University of Rome Tor Vergata, 00133 Rome, Italy
Cassandra Troiano: Department of Chemical Sciences and Technology, University of Rome Tor Vergata, 00133 Rome, Italy
Hugo van Ingen: NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
Renato Massoud: Department of Experimental Medicine, University of Rome Tor Vergata, Viale della Ricerca Scientifica, 00133 Rome, Italy
Nicoletta Perini: Department of Biology, University of Rome Tor Vergata, 00133 Rome, Italy
Luciana Migliore: Department of Biology, University of Rome Tor Vergata, 00133 Rome, Italy
Philippe Savarin: Chemistry Structures Properties of Biomaterials and Therapeutic Agents Laboratory (CSPBAT), Nanomédecine Biomarqueurs Détection Team (NBD), Sorbonne Paris Nord University, The National Center for Scientific Research (CNRS), UMR 7244, 74 Rue Marcel Cachin, CEDEX, 93017 Bobigny, France
Charles Sanders: Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
Lorenzo Stella: Department of Chemical Sciences and Technology, University of Rome Tor Vergata, 00133 Rome, Italy
Marco Sette: Department of Chemical Sciences and Technology, University of Rome Tor Vergata, 00133 Rome, Italy

DOI: https://doi.org/10.3390/membranes13030365
Journal volume & issue: Vol. 13, no. 3
p. 365

Abstract

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(1) Background: antimicrobial resistance is becoming a dramatic problem for public health, and the design of new antimicrobial agents is an active research area. (2) Methods: based on our previous work, we designed an improved version of the crabrolin peptide and characterized its functional and structural properties with a wide range of techniques. (3) Results: the newly designed peptide, crabrolin21, is much more active than the previous ones and shows specific selectivity towards bacterial cells. (4) Conclusions: crabrolin21 shows interesting properties and deserves further studies.

Published in Membranes

ISSN: 2077-0375 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Chemical engineering
Website: https://www.mdpi.com/journal/membranes

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