H2O2 mediates transcriptome reprogramming during Soybean mosaic virus-induced callose deposition in soybean
Tianjie Sun,
Xizhe Sun,
Fukuan Li,
Nan Ma,
Mengxuan Wang,
Yan Chen,
Na Liu,
Yuan Jin,
Jie Zhang,
Chunyan Hou,
Chunyan Yang,
Dongmei Wang
Affiliations
Tianjie Sun
State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, Hebei, China; Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, Hebei, China; College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China
Xizhe Sun
State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, Hebei, China; Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, Hebei, China; College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China
Fukuan Li
College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China; Market Supervision Administration of Chongli District, Zhangjiakou 076350, Hebei, China
Nan Ma
State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, Hebei, China; Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, Hebei, China; College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China
Mengxuan Wang
State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, Hebei, China; Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, Hebei, China; College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China
Yan Chen
State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, Hebei, China; Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, Hebei, China; College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China
Na Liu
State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, Hebei, China; Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, Hebei, China; College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China
Yuan Jin
College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China
Jie Zhang
State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, Hebei, China; Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, Hebei, China; College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China
Chunyan Hou
State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, Hebei, China; Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, Hebei, China; College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China; Corresponding authors.
Chunyan Yang
Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei, China; Corresponding authors.
Dongmei Wang
State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, Hebei, China; Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, Hebei, China; College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China; Corresponding authors.
The main defense response to Soybean mosaic virus (SMV) infection in soybean [Glycine max (L.) Merr.] is thought to be blockage of intercellular virus transport by callose deposition on plasmodesmata. But the specific regulatory mechanism remains largely unknown. In this study, we found that hydrogen peroxide (H2O2) signal downstream of NO was associated with the regulation of callose accumulation. Abundant H2O2 was produced on the cell membrane and cell wall in the incompatible combination of soybean cultivar Jidou 7 and SMV strain N3, whereas no obvious H2O2 was observed in the compatible combination of Jidou 7 and strain SC-8. When H2O2 production was inhibited, callose accumulation induced by SMV infection decreased to a level insufficient to restrict virus transport in the incompatible combination. The H2O2-associated transcriptome dynamics of soybean during SMV infection was investigated. Transcriptome and functional analysis using virus-induced gene silencing showed that GmSEOB and GmPAP27, two genes regulated by H2O2, functioned in resistance by positively regulating the accumulation of callose in response to SMV infection. These results lay a foundation for further research on the signal transduction and molecular regulation of callose deposition during soybean resistance to SMV infection.
