Nature Communications (Dec 2019)
High-resolution label-free 3D mapping of extracellular pH of single living cells
- Yanjun Zhang,
- Yasufumi Takahashi,
- Sung Pil Hong,
- Fengjie Liu,
- Joanna Bednarska,
- Philip S. Goff,
- Pavel Novak,
- Andrew Shevchuk,
- Sahana Gopal,
- Iros Barozzi,
- Luca Magnani,
- Hideki Sakai,
- Yoshimoto Suguru,
- Takuto Fujii,
- Alexander Erofeev,
- Peter Gorelkin,
- Alexander Majouga,
- Dominik J. Weiss,
- Christopher Edwards,
- Aleksandar P. Ivanov,
- David Klenerman,
- Elena V. Sviderskaya,
- Joshua B. Edel,
- Yuri Korchev
Affiliations
- Yanjun Zhang
- Department of Medicine, Imperial College London
- Yasufumi Takahashi
- Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi
- Sung Pil Hong
- Department of Surgery and Cancer, Imperial College London
- Fengjie Liu
- Department of Earth Science & Engineering, Imperial College London
- Joanna Bednarska
- Department of Medicine, Imperial College London
- Philip S. Goff
- Cell Biology Research Centre, Molecular and Clinical Sciences Research Institute, St George’s, University of London
- Pavel Novak
- Department of Medicine, Imperial College London
- Andrew Shevchuk
- Department of Medicine, Imperial College London
- Sahana Gopal
- Department of Medicine, Imperial College London
- Iros Barozzi
- Department of Surgery and Cancer, Imperial College London
- Luca Magnani
- Department of Surgery and Cancer, Imperial College London
- Hideki Sakai
- Department of Pharmaceutical Physiology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
- Yoshimoto Suguru
- Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi
- Takuto Fujii
- Department of Pharmaceutical Physiology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
- Alexander Erofeev
- National University of Science and Technology “MISIS”
- Peter Gorelkin
- National University of Science and Technology “MISIS”
- Alexander Majouga
- Department of Chemistry, Lomonosov Moscow State University
- Dominik J. Weiss
- Department of Earth Science & Engineering, Imperial College London
- Christopher Edwards
- Department of Medicine, Imperial College London
- Aleksandar P. Ivanov
- Department of Chemistry, Imperial College London
- David Klenerman
- Department of Chemistry, University of Cambridge
- Elena V. Sviderskaya
- Cell Biology Research Centre, Molecular and Clinical Sciences Research Institute, St George’s, University of London
- Joshua B. Edel
- Department of Chemistry, Imperial College London
- Yuri Korchev
- Department of Medicine, Imperial College London
- DOI
- https://doi.org/10.1038/s41467-019-13535-1
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 9
Abstract
Current methods to measure extracellular pH are often limited in resolution and response times. Here the authors present a label-free nanoprobe, consisting of a zwitterionic nanomembrane at the tip of a nanopipette, which enables high spatiotemporal resolution pH measurements and topography-pH 3D mapping in live cancer cells.
