Eco-Friendly Synthesis of Functionalized Carbon Nanodots from Cashew Nut Skin Waste for Bioimaging

Abstract

In this study, Anacardium occidentale (A. occidentale) nut skin waste (cashew nut skin waste) was used as a raw material to synthesize functionalized carbon nanodots (F-CNDs). A. occidentale biomass-derived F-CNDs were synthesized at a low temperature (200 °C) using a facile, economical hydrothermal method and subjected to XRD, FESEM, TEM, HRTEM, XPS, Raman Spectroscopy, ATR-FTIR, and Ultraviolet-visible (UV–vis) absorption and fluorescence spectroscopy to determine their structures, chemical compositions, and optical properties. The analysis revealed that dispersed, hydrophilic F-CNDs had a mean diameter of 2.5 nm. XPS and ATR-FTIR showed F-CNDs had a crystalline core and an amorphous surface decorated with –NH2, –COOH, and C=O. In addition, F-CNDs had a quantum yield of 15.5% and exhibited fluorescence with maximum emission at 406 nm when excited at 340 nm. Human colon cancer (HCT-116) cell assays showed that F-CNDs readily penetrated into the cells, had outstanding biocompatibility, high photostability, and minimal toxicity. An MTT assay showed that the viability of HCT-116 cells incubated for 24 h in the presence of F-CNDs (200 μg mL–1) exceeded 95%. Furthermore, when stimulated by filters of three different wavelengths (405, 488, and 555 nm) under a laser scanning confocal microscope, HCT-116 cells containing F-CNDs emitted blue, red, and green, respectively, which suggests F-CNDs might be useful in the biomedical field. Thus, we describe the production of a fluorescent nanoprobe from cashew nut waste potentially suitable for bioimaging applications.

Keywords

• cashew nut skin
• carbon nanodot
• human colon cancer cell
• cell viability
• bioimaging