Immobilization of Candida Rugosa Lipase on Aluminosilicate Incorporated in a Polymeric Membrane for the Elaboration of an Impedimetric Biosensor

Abstract

This work describes the development of a new biosensor for the detection of an organophosphate insecticide (Diazinon) in an aqueous medium. The method is based up on the immobilization of Candida Rugosa Lipase enzyme by adsorption on Aluminosilicate in order to incorporate it into a polymeric membrane. The electrical properties of the polymeric membrane have been characterized by electrochemical impedance spectroscopy (EIS). The linear range of the biosensor is from 10-8 M to 10-6 M with a detection limit of 10-8 M.

Keywords

• Immobilization
• Aluminosilicate
• Biosensor
• Diazinon
• Impedance.