Molecular and Chemical Markers to Illustrate the Complex Diversity of the Genus <i>Lupinus</i> (Fabaceae)
Kalina Bermúdez-Torres,
Maxime Ferval,
Arianna Michelle Hernández-Sánchez,
Andreas Tei,
Charles Gers,
Michael Wink,
Luc Legal
Affiliations
Kalina Bermúdez-Torres
Departamento de Biotecnología, Instituto Politécnico Nacional-Centro de Desarrollo de Productos Bióticos, Yautepec-Jojutla km 8, Calle CEPROBI No. 6 Col. San Isidro, Yautepec C.P. 62731, Mexico
Maxime Ferval
Laboratoire Ecologie Fonctionnelle et Environnement, Université Paul Sabatier, CNRS, 31062 Toulouse, France
Arianna Michelle Hernández-Sánchez
Departamento de Biotecnología, Instituto Politécnico Nacional-Centro de Desarrollo de Productos Bióticos, Yautepec-Jojutla km 8, Calle CEPROBI No. 6 Col. San Isidro, Yautepec C.P. 62731, Mexico
Andreas Tei
Department of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, Germany
Charles Gers
Laboratoire Ecologie Fonctionnelle et Environnement, Université Paul Sabatier, CNRS, 31062 Toulouse, France
Michael Wink
Department of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, INF 364, 69120 Heidelberg, Germany
Luc Legal
Laboratoire Ecologie Fonctionnelle et Environnement, Université Paul Sabatier, CNRS, 31062 Toulouse, France
The potential of secondary metabolites as systematic markers to get new insights in an intricate phylogeny of a recent evolutionary radiation is explored. A chemosystematic study of the genus Lupinus (Fabaceae) was performed, using quinolizidine (QA) and piperidine alkaloids (ammodendrine) as diagnostic characters. Seven major QA and the piperidine alkaloid ammodendrine were found to be the most frequent compounds. Two groups were supported according to their geographic origin: an Old World/Atlantic American group and a West New World group and this pattern is concordant with molecular data (here, based on an original barcode approach using the nuclear marker ITS). However, QA profiles are less informative at the species level. Despite a lack of resolution within the two groups, the alkaloid profiles agree with well supported clades based on DNA molecular characters. The combined use of chemical and barcode genetic markers represents a viable alternative for separating recent evolutionary lineages to a first approximation without having to resort to an expensive and sophisticated molecular arsenal such as next generation sequencing.