Preventive effect of nasal Timosaponin BII-loaded temperature-/ion-sensitive in situ hydrogels on Alzheimer’s disease

Abstract

Objective: To study the preventive effect of Timosaponin BII (T-BII) -loaded temperature/ion-sensitive nasal in situ hydrogels (ISGs) on Alzheimer's disease (AD), its preparation technology, characteristics and in vivo effects were evaluated. Methods: The morphological and rheological properties were evaluated. The preventive effects of T-BII ISG on scopolamine-induced AD in mice were determined with the index of muscarinicreceptor 1 (M1) expression and pathological changes. Results: Results revealed that T-BII ISG significantly increased the content of M1 choline receptors in the hippocampus of mice and ameliorated the damage incurred to the hippocampal cornu ammonis 1 (CA1) area. Conclusion: T-BII ISGs is a reasonable and convenient method of exerting an obvious preventive effect on mice with AD induced by scopolamine. This, thereby, lays forth a new treatment option for preventing AD.

Keywords

• Timosaponin BII
• Alzheimer’s disease
• In situ hydrogels
• Intranasal administration