The impact of particulate matter (PM2.5) on skin barrier revealed by transcriptome analysis: Focusing on cholesterol metabolism

Abstract

Accumulating evidence suggests that particulate matter (PM2.5), as a major air pollutant, imposes a certain degree of destruction and toxicity to the skin. It particularly impairs the structure and function of the epidermis. To study the impact of PM2.5 on the skin, transcriptome analysis was performed on PM2.5-exposed human primary keratinocytes. Functional annotation analysis demonstrates that PM2.5 significantly up-regulates cholesterol-metabolism-related genes. Via lipid extraction from PM2.5 treated three-dimensional epidermis tissue model (3D-ETM) and subsequent characterization via mass spectrometry, it was confirmed that PM2.5 significantly increases epidermal cholesterol levels in vitro. Conversely, the amount of squalene in 3D-ETM was significantly reduced by PM2.5. Interestingly, neither cholesterol nor squalene showed significant fluctuations in the green tea extract (GTE) treated epidermis tissue model under PM2.5 exposure. This study shows that PM2.5 may cause barrier disorders by increasing cholesterol synthesis, leading to transient accumulation of epidermal cholesterol and decrease of squalene. It was suggested that cholesterol and squalene, which are the key substances affecting skin barrier function, can be used as new biomarkers of skin damage induced by PM2.5. Moreover, it was demonstrated that GTE can reduce damage caused by PM2.5 exposure by not only anti-inflammatory and antioxidant mechanisms, but also by off-setting the disturbance to epidermal lipid homeostasis. This study demonstrates the strong potential of GTE as an active ingredient to be utilized in cosmetic products to effectively reduce the damage PM2.5 induces in skin.

Keywords

• PM2.5
• Transcriptome analysis
• Cholesterol metabolism
• Squalene
• 3D-epidermis tissue model
• Green tea extract