Genome-Wide Identification and Characterization of the NAC Gene Family and Its Involvement in Cold Response in <i>Dendrobium officinale</i>

Qianyu Yang; Zhihui Li; Xiao Wang; Chunqian Jiang; Feihong Liu; Yuxin Nian; Xiaoyun Fu; Guangzhu Zhou; Lei Liu; Hui Wang

doi:10.3390/plants12203626

Plants (Oct 2023)

Genome-Wide Identification and Characterization of the NAC Gene Family and Its Involvement in Cold Response in <i>Dendrobium officinale</i>

Qianyu Yang,
Zhihui Li,
Xiao Wang,
Chunqian Jiang,
Feihong Liu,
Yuxin Nian,
Xiaoyun Fu,
Guangzhu Zhou,
Lei Liu,
Hui Wang

Affiliations

Qianyu Yang: College of Forestry, Shenyang Agricultural University, Shenhe District, Shenyang 110866, China
Zhihui Li: College of Forestry, Shenyang Agricultural University, Shenhe District, Shenyang 110866, China
Xiao Wang: College of Forestry, Shenyang Agricultural University, Shenhe District, Shenyang 110866, China
Chunqian Jiang: Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Feihong Liu: College of Forestry, Shenyang Agricultural University, Shenhe District, Shenyang 110866, China
Yuxin Nian: College of Forestry, Shenyang Agricultural University, Shenhe District, Shenyang 110866, China
Xiaoyun Fu: College of Forestry, Shenyang Agricultural University, Shenhe District, Shenyang 110866, China
Guangzhu Zhou: College of Forestry, Shenyang Agricultural University, Shenhe District, Shenyang 110866, China
Lei Liu: Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Hui Wang: Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

DOI: https://doi.org/10.3390/plants12203626
Journal volume & issue: Vol. 12, no. 20
p. 3626

Abstract

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The NAC (NAM, ATAF1/2 and CUC2) gene family is one of the largest plant-specific transcription factor families, functioning as crucial regulators in diverse biological processes such as plant growth and development as well as biotic and abiotic stress responses. Although it has been widely characterized in many plants, the significance of the NAC family in Dendrobium officinale remained elusive up to now. In this study, a genome-wide search method was conducted to identify NAC genes in Dendrobium officinale (DoNACs) and a total of 110 putative DoNACs were obtained. Phylogenetic analysis classified them into 15 subfamilies according to the nomenclature in Arabidopsis and rice. The members in the subfamilies shared more similar gene structures and conversed protein domain compositions. Furthermore, the expression profiles of these DoNACs were investigated in diverse tissues and under cold stress by RNA-seq data. Then, a total of five up-regulated and five down-regulated, cold-responsive DoNACs were validated through QRT-PCR analysis, demonstrating they were involved in regulating cold stress response. Additionally, the subcellular localization of two down-regulated candidates (DoNAC39 and DoNAC58) was demonstrated to be localized in the nuclei. This study reported the genomic organization, protein domain compositions and expression patterns of the NAC family in Dendrobium officinale, which provided targets for further functional studies of DoNACs and also contributed to the dissection of the role of NAC in regulating cold tolerance in Dendrobium officinale.

Published in Plants

ISSN: 2223-7747 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Botany
Website: http://www.mdpi.com/journal/plants

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