Echinococcus granulosus: first report of microcysts formation from protoscoleces of cattle origin using the in vitro vesicular culture technique

Abstract

The aim of this work was the achievement of microcysts formation from protoscoieces of E. granulosus of cattle origin using the in vitro vesicular culture technique. Vesiculated protoscoieces and protoscoieces with posterior bladders appeared during the first week of incubation. After 14 days of culture, a laminated layer appeared like a fine membrane in one of the extremes of the protoscoieces. On day 20, some microcysts with a complete laminated layer were observed. By day 48, microcysts completely developed could be observed. This is the first study where microcysts formation was obtained using protoscoieces of E. granulosus of cattle origin.

Keywords

• Echinococcus granulosus
• cystic echinococcosis
• protoscoleces,
• in vitro culture