Malaria Journal (Jun 2003)

Comparison of <it>Plasmodium falciparum</it> transfection methods

  • Trenholme Katharine R,
  • Gardiner Donald L,
  • Hawthorne Paula L,
  • Lawrie Paula M,
  • Skinner-Adams Tina S

DOI
https://doi.org/10.1186/1475-2875-2-19
Journal volume & issue
Vol. 2, no. 1
p. 19

Abstract

Read online

Abstract The development of an electroporation based transfection method for Plasmodium falciparum has been very successful for the study of some genes but its efficiency remains very low. While alternative approaches have been documented, electroporation of infected red blood cells generally remains the method of choice for introducing DNA into P. falciparum. In this paper we compare four published transfection techniques in their ability to achieve stable transfections.