The spin mixing conductance g↑↓ of a ferromagnetic and a nonmagnetic (NM) layer characterizes the transport efficiency of spin current through the interface. Exploration of the relationship between g↑↓ and structural parameters such as the lattice orientation of the NM layer is critical to design effective spintronics devices. Here, the spin mixing conductance of two types of single oriented Pt and Ni81Fe19 (Py) was studied experimentally, with the method of spin pumping. The obtained g↑↓ for Pt (100)/Py and Pt (111)/Py bilayers is 8.6 ± 0.9 nm−2 and 10.8 ± 1.6 nm−2, respectively, revealing the fact that the crystalline orientation of the metallic NM layer has no prominent impact on g↑↓, which is consistent with the theoretical prediction.