Инфекция и иммунитет (Jul 2019)
Choosing a proper approach for isolating bacteriophages specific to Flavobacterium genus bacteria
Abstract
The article presents results of research related to the selection of an effective method for isolating bacteriophages active against bacteria of the genus Flavobacterium. The number of bacterial strains used in the work — 3. All strains cultures were obtained from the museum of the department of microbiology, virology, epizootiology and veterinary-sanitary examination of federal state budgetary educational institution of higher education Ulyanovsk «State Agricultural University named after PA Stolypin» and have tinctorial and biochemical properties characteristic for this genus (and species). The studies consisted of 4 series of experiments. The first series of experiments was connected with the approbation of a technique for isolating a bacteriophage without using an influencing factor. As a result, the desired bacteriophages were not isolated. The second series of experiments consisted in testing the isolation method using an inducing factorultraviolet irradiation. The distance to the objects of irradiation is0.3 m. Exposure time: 1 min, 3 min, 5 min, 10 min, 15 min. The third series of experiments was also associated with the use of inducing factor X-ray irradiation. In the course of the work, three operating modes were determined, each of which differed in the duration of the exposure, the periodicity of the exposure, and the dose received. As a result of studies related to the use of inducing factors, the desired bacteriophages were not identified. The last series of experiments was to allocate bacteriophages from the objects of the natural places сommemorative (possible) existence of the «owner» (the studied microorganism) and viruses (bacteriophage desired). Three phage isolates were isolated from environmental objects: FL-j-1, FL-pec-2, FL-aq-3. Bacteriophage FL-j-1 is active against a strain of F. johnsoniae VKM B-1426 bacteriophage FL-pec-2 is active against strain F. pectinovorum VKM B-1171 bacteriophage FL-aq-3 strain active against F. aquatile VKPM B-8534. Further studies may be related to study properties of the isolated phages: study of the specificity in respect of this kind of sensitivity study, the study and higher lytic activity, selection of the most promising strains phages further selection of selected phages, as well as studying the possibility of bacteriophages from use in the method of the reaction increase titer phage for the purpose of indicating the microorganism being studied.
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