Histological and Histochemical Dynamism of Oogenesis in the Cinnamon River Prawn Macrobrachium acanthurus (Caridea: Palaemonidae) Induced by Eyestalk Ablation

Abstract

Abstract Macrobrachium acanthurus is a caridean prawn native to Brazil, and studying techniques to assist in its cultivation is important due to economic interest in it. Eyestalk ablation is commonly used to promote ovarian development and maturation of captive crustaceans, but it can have possible consequences on fertility and brood quality. Histological and histochemical dynamism of oogenesis was analyzed under control (non-ablated females) and unilateral eyestalk ablated females. Females with ovaries in the spent stage of gonadal development were divided into two treatments: unilaterally ablated and non-ablated. A second experiment with the same treatments was conducted using females with mature gonads. Histological and histochemical analyses of the ovaries indicated that the ablation did not affect oogenesis based on ovarian structure, oocyte size, histochemical properties, and atretic oocytes. Ovarian maturation was not accelerated by ablation, either. Survival, nuptial molting, and spawning were also unaffected. From an applied point of view, unilateral eyestalk ablation is not needed in M. acanthurus farming because it does not improve reproductive performance.

Keywords

• Maturation
• morphology
• ovary
• vitellogenesis
• X-organ sinus gland complex