Nature Communications (Mar 2022)
Rapid expansion and extinction of antibiotic resistance mutations during treatment of acute bacterial respiratory infections
- Hattie Chung,
- Christina Merakou,
- Matthew M. Schaefers,
- Kelly B. Flett,
- Sarah Martini,
- Roger Lu,
- Jennifer A. Blumenthal,
- Shanice S. Webster,
- Ashley R. Cross,
- Roy Al Ahmar,
- Erin Halpin,
- Michelle Anderson,
- Nicholas S. Moore,
- Eric C. Snesrud,
- Hongwei D. Yu,
- Joanna B. Goldberg,
- George A. O’Toole,
- Patrick McGann,
- Jason A. Stam,
- Mary Hinkle,
- Alexander J. McAdam,
- Roy Kishony,
- Gregory P. Priebe
Affiliations
- Hattie Chung
- Department of Systems Biology, Harvard Medical School
- Christina Merakou
- Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children’s Hospital
- Matthew M. Schaefers
- Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children’s Hospital
- Kelly B. Flett
- Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children’s Hospital
- Sarah Martini
- Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children’s Hospital
- Roger Lu
- Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children’s Hospital
- Jennifer A. Blumenthal
- Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children’s Hospital
- Shanice S. Webster
- Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth
- Ashley R. Cross
- Division of Pulmonary, Allergy & Immunology, Cystic Fibrosis and Sleep, Emory Children’s Center for Cystic Fibrosis and Airway Disease Research, Department of Pediatrics, Emory University School of Medicine
- Roy Al Ahmar
- Department of Biomedical Science, Joan C. Edwards School of Medicine at Marshall University
- Erin Halpin
- Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children’s Hospital
- Michelle Anderson
- Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children’s Hospital
- Nicholas S. Moore
- Harvard Medical School
- Eric C. Snesrud
- Walter Reed Army Institute of Research
- Hongwei D. Yu
- Department of Biomedical Science, Joan C. Edwards School of Medicine at Marshall University
- Joanna B. Goldberg
- Division of Pulmonary, Allergy & Immunology, Cystic Fibrosis and Sleep, Emory Children’s Center for Cystic Fibrosis and Airway Disease Research, Department of Pediatrics, Emory University School of Medicine
- George A. O’Toole
- Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth
- Patrick McGann
- Walter Reed Army Institute of Research
- Jason A. Stam
- Walter Reed Army Institute of Research
- Mary Hinkle
- Walter Reed Army Institute of Research
- Alexander J. McAdam
- Department of Laboratory Medicine, Boston Children’s Hospital
- Roy Kishony
- Department of Systems Biology, Harvard Medical School
- Gregory P. Priebe
- Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children’s Hospital
- DOI
- https://doi.org/10.1038/s41467-022-28188-w
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 10
Abstract
It remains unclear how rapid antibiotic switching affects the evolution of antibiotic resistance in individual patients. Here, Chung et al. combine short- and long-read sequencing and resistance phenotyping of 420 serial isolates of Pseudomonas aeruginosa collected from the onset of respiratory infection, and show that rare resistance mutations can increase by nearly 40-fold over 5–12 days in response to antibiotic changes, while mutations conferring resistance to antibiotics not administered diminish and even go to extinction.
