Jichu yixue yu linchuang (Mar 2022)

Sulforaphane alleviates ischemia- reperfusion injury of heart transplantation in rat models

  • WANG Shuai, WANG Jian-jun, SUN Xiu-hong, WU Peng-yu, CUI Zhi-cheng, LI Zhan-qing

DOI
https://doi.org/10.16352/j.issn.1001-6325.2022.03.029
Journal volume & issue
Vol. 42, no. 3
pp. 411 – 416

Abstract

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Objective To investigate the effect of sulforaphane on ischemia-reperfusion injury(I/RI) and the role of PI3K/Akt/GSK-3β pathway in heart transplantation of rats. Methods The rat model of allograft heart transplantation was established as the control group.The survival rate,the level of serum myocardial enzymes,apoptosis of myocardial cells and the swelling degree of myocardial mitochondria were compared with sulforaphane injection group (2.5 mg/kg).Western blot was used to detect the expression of apoptotic molecules caspase-3,Bax and PI3K/Akt/GSK-3β. Results The survival rate of sulforaphane group was significantly higher than that of control group(P＜0.05).The serum concentrations of LDH,CK,CK-MB and TNT in sulforaphane group were lower than those in control group with a time dependent manner at different treatment time (6,12,24 and 48 h).The expressions of caspase-3 and Bax were significantly decreased (P＜0.01),and the degree of mitochondrial swelling was also improved compared with the control group. Conclusions Sulforaphane may inhibit myocardial apoptosis by activating PI3K/Akt/GSK-3β pathway and improve I/RI after heart transplantation as shown by animal model.

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