Effects of replacing fish meal with corn gluten meal on growth performance, intestinal microbiota, mTOR pathway and immune response of abalone Haliotis discus hannai

Zhenhua Wu; Xiaojun Yu; Jinshu Guo; Yonghao Fu; Yanlin Guo; Mingzhu Pan; Wenbing Zhang; Kangsen Mai

Aquaculture Reports (Apr 2022)

Effects of replacing fish meal with corn gluten meal on growth performance, intestinal microbiota, mTOR pathway and immune response of abalone Haliotis discus hannai

Zhenhua Wu,
Xiaojun Yu,
Jinshu Guo,
Yonghao Fu,
Yanlin Guo,
Mingzhu Pan,
Wenbing Zhang,
Kangsen Mai

Affiliations

Zhenhua Wu: The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), Fisheries College, Ocean University of China, Qingdao 266003, China; The Key Laboratory of Mariculture (Ministry of Education), Fisheries College, Ocean University of China, Qingdao 266003, China
Xiaojun Yu: The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), Fisheries College, Ocean University of China, Qingdao 266003, China; The Key Laboratory of Mariculture (Ministry of Education), Fisheries College, Ocean University of China, Qingdao 266003, China
Jinshu Guo: The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), Fisheries College, Ocean University of China, Qingdao 266003, China; The Key Laboratory of Mariculture (Ministry of Education), Fisheries College, Ocean University of China, Qingdao 266003, China
Yonghao Fu: The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), Fisheries College, Ocean University of China, Qingdao 266003, China; The Key Laboratory of Mariculture (Ministry of Education), Fisheries College, Ocean University of China, Qingdao 266003, China
Yanlin Guo: The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), Fisheries College, Ocean University of China, Qingdao 266003, China; The Key Laboratory of Mariculture (Ministry of Education), Fisheries College, Ocean University of China, Qingdao 266003, China
Mingzhu Pan: The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), Fisheries College, Ocean University of China, Qingdao 266003, China; The Key Laboratory of Mariculture (Ministry of Education), Fisheries College, Ocean University of China, Qingdao 266003, China
Wenbing Zhang: The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), Fisheries College, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Wen Hai Road, Qingdao 266237, China; The Key Laboratory of Mariculture (Ministry of Education), Fisheries College, Ocean University of China, Qingdao 266003, China; Correspondence to: Fisheries College, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.
Kangsen Mai: The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), Fisheries College, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Wen Hai Road, Qingdao 266237, China; The Key Laboratory of Mariculture (Ministry of Education), Fisheries College, Ocean University of China, Qingdao 266003, China

Journal volume & issue: Vol. 23
p. 101007

Abstract

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Corn gluten meal (CGM) is used as a protein source in aquafeeds. However, the potential for replacing fish meal (FM) with CGM in abalone feeds has not been adequately evaluated. A 110-day feeding trial was conducted to evaluate effects of replacing FM with CGM on abalone Haliotis discus hannai (initial weight: 21.83 ± 0.25 g). The control diet (CGM0) was designed to contain 20% of FM. The other four isonitrogenous (about 30% of crude protein) and isolipidic (about 4% of crude lipid) experimental diets were formulated with CGM protein replacing 25%, 50%, 75% and 100% of dietary FM protein, respectively. The five experimental diets were named as CGM0, CGM25, CGM50, CGM75 and CGM100, respectively. Results showed that no significant differences were found in survival rate among all the groups. Weight gain rate (WGR) of abalone in the CGM100 group was significantly lower than that in the control group. There were no significant differences in WGR among the CGM25, CGM50, CGM75 and the control groups. The increasing dietary CGM inclusion level elevated the operational taxonomic units, ACE estimator and Chao1 estimator, and changed the intestinal microbiota composition in abalone. The activities of trypsin, alanine transaminase, aspartate transaminase and Na+, K+-ATPase in digestive gland were significantly decreased when replacement level over 50%. The increasing dietary CGM inclusion level significantly downregulated the expressions of ribosomal S6 protein kinase (s6k), mammalian target of rapamycin (mtor) and eukaryotic translation initiation factor 4E (eif4e) in digestive gland. The CGM75 and CGM100 groups had significant lower activities of catalase and lysozyme in cell-free hemolymph and digestive gland. In conclusion, CGM could replace 75% of dietary FM (equivalent to 16.2% of CGM inclusion level in the diet) without significantly affecting the growth of abalone. However, high inclusion levels of CGM in diets (> 10.8%) had negative effects on intestinal microbiota, mTOR pathway and immunity in abalone.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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