A Dual pH/O<sub>2</sub> Sensing Film Based on Functionalized Electrospun Nanofibers for Real-Time Monitoring of Cellular Metabolism
Dongyan Zhou,
Hongtian Liu,
Juewei Ning,
Ge Cao,
He Zhang,
Mengyu Deng,
Yanqing Tian
Affiliations
Dongyan Zhou
Department of Materials Science and Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Blvd., Xili, Nanshan District, Shenzhen 518055, China
Hongtian Liu
Department of Materials Science and Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Blvd., Xili, Nanshan District, Shenzhen 518055, China
Juewei Ning
Department of Materials Science and Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Blvd., Xili, Nanshan District, Shenzhen 518055, China
Ge Cao
Department of Materials Science and Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Blvd., Xili, Nanshan District, Shenzhen 518055, China
He Zhang
Department of Materials Science and Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Blvd., Xili, Nanshan District, Shenzhen 518055, China
Mengyu Deng
Department of Materials Science and Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Blvd., Xili, Nanshan District, Shenzhen 518055, China
Yanqing Tian
Department of Materials Science and Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Blvd., Xili, Nanshan District, Shenzhen 518055, China
Real-time monitoring of dissolved oxygen (DO) and pH is of great significance for understanding cellular metabolism. Herein, a dual optical pH/O2 sensing membrane was prepared by the electrospinning method. Cellulose acetate (CA) and poly(ε-caprolactone) (PCL) nanofiber membrane blended with platinum (II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorophenyl)-porphyrin (PtTFPP) was used as the DO sensing matrix, upon which electrospun nanofiber membrane of chitosan (CS) coupled with fluorescein 5-isothiocyanate (FITC) was used as the pH sensing matrix. The electrospun sensing film prepared from biocompatible biomaterials presented good response to a wide range of DO concentrations and physiological pH. We used it to monitor the exracellular acidification and oxygen consumption levels of cells and bacteria. This sensing film can provide a luminescence signal change as the DO and pH change in the growth microenvironment. Due to its advantages of good biocompatibility and high stability, we believe that the dual functional film has a high value in the field of biotechnology research.
