Antimicrobial Essential Oil Formulation: Chitosan Coated Nanoemulsions for Nose to Brain Delivery

Federica Rinaldi; Alessandra Oliva; Manuela Sabatino; Anna Imbriano; Patrizia N. Hanieh; Stefania Garzoli; Claudio M. Mastroianni; Massimiliano De Angelis; Maria Claudia Miele; Marcela Arnaut; Federica Di Timoteo; Carlotta Marianecci; Rino Ragno; Maria Carafa

doi:10.3390/pharmaceutics12070678

Pharmaceutics (Jul 2020)

Antimicrobial Essential Oil Formulation: Chitosan Coated Nanoemulsions for Nose to Brain Delivery

Federica Rinaldi,
Alessandra Oliva,
Manuela Sabatino,
Anna Imbriano,
Patrizia N. Hanieh,
Stefania Garzoli,
Claudio M. Mastroianni,
Massimiliano De Angelis,
Maria Claudia Miele,
Marcela Arnaut,
Federica Di Timoteo,
Carlotta Marianecci,
Rino Ragno,
Maria Carafa

Affiliations

Federica Rinaldi: Department of Drug Chemistry and Technology, Sapienza University of Rome, 00185 Rome, Italy
Alessandra Oliva: Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy
Manuela Sabatino: Department of Drug Chemistry and Technology, Rome Center for Molecular Design, 00185 Rome, Italy
Anna Imbriano: Department of Drug Chemistry and Technology, Sapienza University of Rome, 00185 Rome, Italy
Patrizia N. Hanieh: Department of Drug Chemistry and Technology, Sapienza University of Rome, 00185 Rome, Italy
Stefania Garzoli: Department of Drug Chemistry and Technology, Sapienza University of Rome, 00185 Rome, Italy
Claudio M. Mastroianni: Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy
Massimiliano De Angelis: Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy
Maria Claudia Miele: Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy
Marcela Arnaut: Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy
Federica Di Timoteo: Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy
Carlotta Marianecci: Department of Drug Chemistry and Technology, Sapienza University of Rome, 00185 Rome, Italy
Rino Ragno: Department of Drug Chemistry and Technology, Sapienza University of Rome, 00185 Rome, Italy
Maria Carafa: Department of Drug Chemistry and Technology, Sapienza University of Rome, 00185 Rome, Italy

DOI: https://doi.org/10.3390/pharmaceutics12070678
Journal volume & issue: Vol. 12, no. 7
p. 678

Abstract

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Brain infections as meningitis and encephalitis are attracting a great interest. Challenges in the treatment of these diseases are mainly represented by the blood brain barrier (BBB) that impairs the efficient delivery of even very potent drugs to reach the brain. The nose to the brain administration route, is a non-invasive alternative for a quick onset of action, and enables the transport of numerous medicinal agents straight to the brain thus workarounding the BBB through the highly vascularized olfactory region. In this report, Thymus vulgaris and Syzygium aromaticum essential oils (EOs) were selected to be included in chitosan coated nanoemulsions (NEs). The EOs were firstly analyzed to determine their chemical composition, then used to prepare NEs, that were deeply characterized in order to evaluate their use in intranasal administration. An in vitro evaluation against a collection of clinical isolated bacterial strains was carried out for both free and nanoemulsioned EOs. Chitosan coated NEs showed to be a potential and effective intranasal formulation against multi-drug resistant Gram-negative bacteria such as methicillin-susceptible Staphylococcus aureus and multi-drug resistant Gram-negative microorganisms including carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae.

Published in Pharmaceutics

ISSN: 1999-4923 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Pharmacy and materia medica
Website: http://www.mdpi.com/journal/pharmaceutics/

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