Morphology and immunohistochemistry analysis of broiler intestine treated with immunostimulant agents

Abstract

This study aims to examine the effects of Saccharomyces cerevisiae and a probiotic, mixing them on the intestine layer represented by (heights of the epithelium, depth of the crypts, width of villus and length of villi), and to observe the histopathological and immunohistochemical effects in broilers during 1-35 days. A total of sixty birds 1-day old broilers are randomly divided into four groups each with fifteen replicates, where G1 as a control group, G2 treated with 3 g/kg of S. cerevisiae, G3 treated with 200g/1000L of probiotics with water and G4 treated with a mixture of both probiotics and S.C. All broiler chickens are euthanized at days 7, 21 and 35 for histopathology and at days 7, 35 for immunohistochemistry. Results show a significant variation increase in the intestine layer of all groups in contrast to the control group, especially at G4 (the mixture of S. cerevisiae and probiotics). The administration of S. cerevisiae and probiotics significantly increases the permeability of the intestine via IL-6 proteins for cytoplasm and cell membrane and the gene expression is equal in intensity during the first and the fifth weeks of killing. From these results, we conclude that the materials used in G4 has given intense response of IL-6 in broiler chickens, which is a good indicator; therefore, they are proposed for stimulating innate and adaptive immunity.

Keywords

• il-6
• gene expression
• probiotics
• s. cerevisiae
• villus