Evaluation of the Anti-Inflammatory and Antioxidant Potential of <i>Cymbopogon citratus</i> Essential Oil in Zebrafish
Kiara Cândido Duarte da Silva,
William Franco Carneiro,
Bárbara do Carmo Rodrigues Virote,
Maria de Fátima Santos,
João Paulo Lima de Oliveira,
Tássia Flávia Dias Castro,
Suzan Kelly Vilela Bertolucci,
Luis David Solis Murgas
Affiliations
Kiara Cândido Duarte da Silva
Faculty of Animal Science and Veterinary Medicine (FZMV), Department of Veterinary Medicine, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil
William Franco Carneiro
Faculty of Animal Science and Veterinary Medicine (FZMV), Department of Veterinary Medicine, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil
Bárbara do Carmo Rodrigues Virote
Faculty of Animal Science and Veterinary Medicine (FZMV), Department of Veterinary Medicine, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil
Maria de Fátima Santos
School of Agricultural Sciences of Lavras (ESAL), Department of Agriculture, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil
João Paulo Lima de Oliveira
School of Agricultural Sciences of Lavras (ESAL), Department of Agriculture, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil
Tássia Flávia Dias Castro
Institute of Biomedical Sciences II (ICBII), Universidade de São Paulo, São Paulo 05508-000, São Paulo, Brazil
Suzan Kelly Vilela Bertolucci
School of Agricultural Sciences of Lavras (ESAL), Department of Agriculture, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil
Luis David Solis Murgas
Faculty of Animal Science and Veterinary Medicine (FZMV), Department of Veterinary Medicine, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil
This study explored the protective capacity of the essential oil (EO) of Cymbopogon citratus against oxidative stress induced by hydrogen peroxide (H2O2) and the inflammatory potential in zebrafish. Using five concentrations of EO (0.39, 0.78, 1.56, 3.12, and 6.25 μg/mL) in the presence of 7.5 mM H2O2, we analyzed the effects on neutrophil migration, caudal fin regeneration, cellular apoptosis, production of reactive oxygen species (ROS), and activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) after 96 h of exposure. A significant decrease in neutrophil migration was observed in all EO treatments compared to the control. Higher concentrations of EO (3.12 and 6.25 μg/mL) resulted in a significant decrease in caudal fin regeneration compared to the control. SOD activity was reduced at all EO concentrations, CAT activity significantly decreased at 3.12 μg/mL, and GST activity increased at 0.78 μg/mL and 1.56 μg/mL, compared to the control group. No significant changes in ROS production were detected. A reduction in cellular apoptosis was evident at all EO concentrations, suggesting that C. citratus EO exhibits anti-inflammatory properties, influences regenerative processes, and protects against oxidative stress and apoptosis.
