Generation of IBMS-iPSC-021, -022, -023 human induced pluripotent stem cells (IBMSi016-A, IBMSi017-A, and IBMSi018-A) derived from patients with the ALDH2 rs671 polymorphism

Ming-Heng Tsai; Yueh-Ting Chiu; Darien Zhing-Herr Chan; Cheng-Hao Wen; Shih-Han Syu; Huai-En Lu; Chi-Ying F. Huang; Yenn-Jiang Lin; Shih-Lin Chang; Li-Wei Lo; Ching-Ying Huang; Yu-Feng Hu; Patrick C.H. Hsieh; Shih-Ann Chen

Stem Cell Research (Jul 2021)

Generation of IBMS-iPSC-021, -022, -023 human induced pluripotent stem cells (IBMSi016-A, IBMSi017-A, and IBMSi018-A) derived from patients with the ALDH2 rs671 polymorphism

Ming-Heng Tsai,
Yueh-Ting Chiu,
Darien Zhing-Herr Chan,
Cheng-Hao Wen,
Shih-Han Syu,
Huai-En Lu,
Chi-Ying F. Huang,
Yenn-Jiang Lin,
Shih-Lin Chang,
Li-Wei Lo,
Ching-Ying Huang,
Yu-Feng Hu,
Patrick C.H. Hsieh,
Shih-Ann Chen

Affiliations

Ming-Heng Tsai: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Yueh-Ting Chiu: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Darien Zhing-Herr Chan: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Cheng-Hao Wen: Food Industry Research and Development Institute, Hsinchu, Taiwan
Shih-Han Syu: Food Industry Research and Development Institute, Hsinchu, Taiwan
Huai-En Lu: Food Industry Research and Development Institute, Hsinchu, Taiwan
Chi-Ying F. Huang: Institute of Biopharmaceutical Sciences, National Yang Ming University, Taipei, Taiwan
Yenn-Jiang Lin: Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; Heart Rhythm Center, Division of Cardiology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan
Shih-Lin Chang: Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; Heart Rhythm Center, Division of Cardiology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan
Li-Wei Lo: Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; Heart Rhythm Center, Division of Cardiology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan
Ching-Ying Huang: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Yu-Feng Hu: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan; Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; Heart Rhythm Center, Division of Cardiology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Corresponding authors.
Patrick C.H. Hsieh: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan; Corresponding authors.
Shih-Ann Chen: Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; Heart Rhythm Center, Division of Cardiology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan

Journal volume & issue: Vol. 54
p. 102416

Abstract

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ALDH2 gene is coded for the aldehyde dehydrogenase (ALDH), which is an enzyme involved in alcohol metabolism. Compared to normal aldehyde dehydrogenases, a homozygous point mutation on exon 12 from G to A significantly reduces its efficiency. In this study, we have reported the generation of IBMS-iPSC-021-04, IBMS-iPSC-022-01, and IBMS-iPSC-023-03 as induced pluripotent stem cell (iPSC) lines carrying the homozygous form of ALDH2 with the rs671 genetic polymorphism (E487K mutation). These cell lines were characterized in terms of pluripotency and differentiation potential. They serve as useful platforms to study alcohol metabolism and other chronic diseases associated with alcohol consumption.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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