Association assessment of Interleukine-10 gene polymorphism and its expression status with susceptibility to coronary artery disease in Iran

Abstract

Background: The cytokines are potent inflammatory factors that regulate each stage of atherosclerosis leading to the disease development. Interleukin-10 (IL-10) as an anti-inflammatory cytokine can develop atherosclerosis by inhibiting the synthesis of metalloproteinase. Moreover, IL-10 promotes the plaque stability by preserving the extracellular matrix and fibrous cap. Aim: We evaluated the association of the two IL-10 promoter gene polymorphisms with susceptibility to coronary artery disease (CAD) in Iranian population. Subjects and methods: We used the Sequence Specific Primer-Polymerase Chain Reaction method to determine genotypes. We also studied mRNA expression of the IL-10 gene in Iranian CAD patients using quantitative real-time PCR. Results: There was a significant association between IL-10(−819) T allele and IL-10(−819) T/T genotype, and CAD (p = 0.041 and p = 0.042 respectively). There also was a significant association between IL-10(−1082) G allele and IL-10(−1082) G/G genotype, and CAD (p = 0.017 and p = 0.020 respectively). Genotype T/T of IL-10(−819) polymorphism significantly associated with the two vessel disease type (p = 0.017). In addition, there was a significant association between IL-10(−1082) G/G genotype and the three vessel disease type (p = 0.009). IL-10 mRNA expression was significantly decreased 3.36-fold in samples with IL-10(−819) polymorphism and 1.98-fold in individuals with IL-10(−1082) polymorphism. Conclusions: Our results suggest that IL-10 gene promoter polymorphisms may influence both coronary artery disease risks and severity in Iranian patients.

Keywords

• Coronary artery disease
• Interleukin-10
• SSP-PCR
• Polymorphism