Nature Communications (Dec 2022)
Sequence-specific capture and concentration of viral RNA by type III CRISPR system enhances diagnostic
- Anna Nemudraia,
- Artem Nemudryi,
- Murat Buyukyoruk,
- Andrew M. Scherffius,
- Trevor Zahl,
- Tanner Wiegand,
- Shishir Pandey,
- Joseph E. Nichols,
- Laina N. Hall,
- Aidan McVey,
- Helen H. Lee,
- Royce A. Wilkinson,
- Laura R. Snyder,
- Joshua D. Jones,
- Kristin S. Koutmou,
- Andrew Santiago-Frangos,
- Blake Wiedenheft
Affiliations
- Anna Nemudraia
- Department of Microbiology and Cell Biology, Montana State University
- Artem Nemudryi
- Department of Microbiology and Cell Biology, Montana State University
- Murat Buyukyoruk
- Department of Microbiology and Cell Biology, Montana State University
- Andrew M. Scherffius
- Department of Microbiology and Cell Biology, Montana State University
- Trevor Zahl
- Department of Microbiology and Cell Biology, Montana State University
- Tanner Wiegand
- Department of Microbiology and Cell Biology, Montana State University
- Shishir Pandey
- Department of Microbiology and Cell Biology, Montana State University
- Joseph E. Nichols
- Department of Microbiology and Cell Biology, Montana State University
- Laina N. Hall
- Department of Microbiology and Cell Biology, Montana State University
- Aidan McVey
- Department of Microbiology and Cell Biology, Montana State University
- Helen H. Lee
- Department of Microbiology and Cell Biology, Montana State University
- Royce A. Wilkinson
- Department of Microbiology and Cell Biology, Montana State University
- Laura R. Snyder
- Department of Chemistry, University of Michigan
- Joshua D. Jones
- Department of Chemistry, University of Michigan
- Kristin S. Koutmou
- Department of Chemistry, University of Michigan
- Andrew Santiago-Frangos
- Department of Microbiology and Cell Biology, Montana State University
- Blake Wiedenheft
- Department of Microbiology and Cell Biology, Montana State University
- DOI
- https://doi.org/10.1038/s41467-022-35445-5
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 12
Abstract
Many viral diagnostic approaches require nucleic acid extraction and amplification prior to detection. Here, the authors develop a method based on type III CRISPR systems which allows sequence specific capture, concentration, and detection of viral RNA directly from patient samples.
