Analysis of Stability of Plague Chemical Vaccine (F1+MSA) Antigenic Components in the Tests of Accelerated Ageing and Stress Conditions

Abstract

Carried out was comprehensive immunochemical and immunobiological analysis of plague microbe species-specific capsule F1 protein, and glycoproteid main somatic antigen (MSA) the latter being common in specificity in closely related plague and pseudotuberculosis agents. Both preparations isolated from Yersinia pestis cells using standard techniques were examined in the test of accelerated ageing (exposure at 37 °C for 4 weeks) and in stress conditions (exposure at 60 °C for 24 hours). They were shown to preserve their main features that characterized their authenticity and specific activity (visual appearance, solubility, chemical content, immunochemical homogeneity and immunobiological activity) as compared with preparations stored in refrigerator at 4-8 °C.

Keywords

• yersinia pestis
• f1 antigen
• msa
• immunochemical and immunobiological properties
• accelerated test of termostability