Dihydroartemisinin inhibits HNSCC invasion and migration by controlling miR-195-5p expression
Xiaolu Wu,
Congwen Yang,
Zhongwan Li,
Ping Lv,
Xiang An,
Xiaohe Peng,
You Li,
Xiaojun Jiang,
Xuemei Mao,
Donghong Chen,
Lifeng Jia,
Wei Yuan
Affiliations
Xiaolu Wu
North Sichuan Medical College (University), Nanchong, China; Department of Otorhinolaryngology-Head and Neck Surgery, Chongqing General Hospital, Chongqing University, Chongqing, China
Congwen Yang
College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing, China
Zhongwan Li
Department of Otorhinolaryngology-Head and Neck Surgery, Chongqing General Hospital, Chongqing University, Chongqing, China
Ping Lv
Affiliated Hospital of North Sichuan Medical College, Nanchong, China; North Sichuan Medical College (University), Nanchong, China
Xiang An
Department of Otorhinolaryngology-Head and Neck Surgery, Chongqing General Hospital, Chongqing University, Chongqing, China
Xiaohe Peng
College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing, China
You Li
College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing, China
Xiaojun Jiang
Chongqing Fifth People's Hospital, Chongqing, China
Xuemei Mao
University-Town Hospital of Chongqing Medical University, Chongqing, China
Donghong Chen
Department of Otorhinolaryngology-Head and Neck Surgery, Chongqing General Hospital, Chongqing University, Chongqing, China
Lifeng Jia
Department of Otorhinolaryngology-Head and Neck Surgery, Chongqing General Hospital, Chongqing University, Chongqing, China; Corresponding author.
Wei Yuan
Department of Otorhinolaryngology-Head and Neck Surgery, Chongqing General Hospital, Chongqing University, Chongqing, China; North Sichuan Medical College (University), Nanchong, China; Corresponding author. Department of Otorhinolaryngology-Head and Neck Surgery, Chongqing General Hospital, Chongqing University, Chongqing, China.
Objectives: Dihydroartemisinin (DHA), an artemisinin derivative extracted from the traditional Chinese medicinal herb Artemisia annua, has the potential to suppress head and neck squamous cell carcinoma (HNSCC) progression. However, the mechanisms underlying these effects remain unclear. Therefore, we aimed to examine the mechanisms underlying the effects of DHA on tumor invasion and migration. Methods: Human HNSCC cell lines CAL-27 and FaDu were exposed to varying DHA concentrations (0, 5, 20, and 80 μM) for 24 h. Cell proliferation, invasion, and migration were assessed using CCK8, transwell, and wound-healing assays, respectively. Quantitative real-time PCR, western blotting, and immunofluorescence were used to assess the expression levels of the target genes and proteins. Results: DHA suppressed the invasion and migration of CAL-27 and FaDu cells. Additionally, miR-195-5p suppressed the invasion and migration of HNSCC cells. This study revealed significant differences in the expression of miR-195-5p and TENM2 between clinical samples and multiple public databases. DHA treatment and miR-195-5p overexpression significantly reduced TENM2 expression in HNSCC cells, which suggested that miR-195-5p overexpression enhanced the inhibitory effect of DHA on TENM2. Conclusions: This study provides the first evidence that DHA inhibits cell invasion and migration by regulating the miR-195-5p/TENM2 axis in HNSCC cells, suggesting it as a potentially effective treatment strategy for HNSCC.
