Method Validation for the Determination of Glyphosate and Aminomethylphosphonic Acid in Water by LC-MS/MS

Abstract

The massive use of glyphosate as a herbicide compound has led to the ubiquity of AMPA in the environment, especially in water. So, the present work aimed to establish the validation parameters of the LC-MS/MS method for glyphosate and its metabolite AMPA, determination in water. During the extraction process the derivatization of glyphosate as an extreme polar pesticide, was done using FMOC. The validation results show that the method met the criteria given in SANTE/11312/2021 for all the investigated validation parameters as well as estimated measurement uncertainty.