Curcumin and vitamin C improve immunity of kidney via gene expression against diethyl nitrosamine induced nephrotoxicity in rats:In vivo and molecular docking studies
Ahmed B.M. Mehany,
Islam M. Farrag,
Mahmoud Diab,
Mohammed M. Ghoneim,
Mohamed El-Sherbiny,
Rasha Hamed Al-Serwi,
Ali H. Amin,
Amany Belal,
Salwa Shaaban,
Alsayed A. Abdelhady
Affiliations
Ahmed B.M. Mehany
Department of zoology Faculty of science Al-Azhar University Cairo, Egypt; Corresponding author.
Islam M. Farrag
Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine (Girls), Al-Azhar University, Cairo, Egypt
Mahmoud Diab
Anatomy and embryology Department faculty of medicine Al-Azhar university,cairo, Egypt; Basic medical science, Faculty of applied medical sciences, university of tabuk saudi arabia
Mohammed M. Ghoneim
Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Ad Diriyah13713, Saudi Arabia; Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt
Mohamed El-Sherbiny
Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 71666, Riyadh, 11597, Saudi Arabia; Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Rasha Hamed Al-Serwi
Department of Basic Dental Sciences, College of Dentistry, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Ali H. Amin
Deanship of Scientific Research, Umm Al-Qura University, Makkah 21955, Saudi Arabia
Amany Belal
Medicinal Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt; Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099,Taif 21944, Saudi Arabia
Salwa Shaaban
Department of Microbiology & Immunology, Faculty of pharmacySuef University, Beni-Suef, Egypt; Department of Clinical Laboratory Sciences, Faculty of Applied medical Sciences, King Khalid University, Abha, Saudi Arabia
Alsayed A. Abdelhady
Department of Anatomy and Embryology, Faculty of Medicine, Helwan University, Helwan, Egypt
Kidney has a crucial role in immunity, so any toxicity occurs for the kidney will result in reduced immunity. The aim of this study is to improve the immune response of insufficient kidneys through immune-related genes. Diethyl Nitrosamine has been used to cause kidney damage in animal models, vitamin C and curcumin have been used to treat impaired kidney. Renal function (urea, uric acid and creatinine) and oxidative stress parameters (superoxide dismutase, malondialdehyde and glutathione peroxidase) will be evaluated in this research work. Molecular docking also will be performed to investigate the role of vitamin C and curcumin in targeting immune response proteins. Also, Complementary component 3, Lipocalin-2, Toll-like receptor 2,Toll-like receptor 4, Kidney injury molecule-1, Interleukin 6, Interleukin-10, Tumor necrosis factor and p38 mitogen-activated protein kinases will be investigated. The obtained results showed that vitamin C and curcumin have good effects in the treatment of impaired kidneys, this was also observed in renal function and oxidative stress parameters, expression levels of proteins and histopathological examinations.
