[18F]Fluoro-DCP, a first generation PET radiotracer for monitoring protein sulfenylation in vivo

Kiran Kumar Solingapuram Sai; Xiaofei Chen; Zhe Li; Caigang Zhu; Kirtikar Shukla; Tom E. Forshaw; Hanzhi Wu; Stephen A. Vance; Buddhika Liyana Pathirannahel; Megan Madonna; Mark W. Dewhirst; Allen W. Tsang; Leslie B. Poole; Nimmi Ramanujam; S. Bruce King; Cristina M. Furdui

Redox Biology (Feb 2022)

[18F]Fluoro-DCP, a first generation PET radiotracer for monitoring protein sulfenylation in vivo

Kiran Kumar Solingapuram Sai,
Xiaofei Chen,
Zhe Li,
Caigang Zhu,
Kirtikar Shukla,
Tom E. Forshaw,
Hanzhi Wu,
Stephen A. Vance,
Buddhika Liyana Pathirannahel,
Megan Madonna,
Mark W. Dewhirst,
Allen W. Tsang,
Leslie B. Poole,
Nimmi Ramanujam,
S. Bruce King,
Cristina M. Furdui

Affiliations

Kiran Kumar Solingapuram Sai: Department of Radiology, Wake Forest School of Medicine, Winston‐Salem, North Carolina, USA
Xiaofei Chen: Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA
Zhe Li: Department of Chemistry, Wake Forest University, Winston-Salem, North Carolina, USA
Caigang Zhu: Department of Biomedical Engineering, Duke University, Durham, NC, USA
Kirtikar Shukla: Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA
Tom E. Forshaw: Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA
Hanzhi Wu: Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA
Stephen A. Vance: Department of Chemistry, Wake Forest University, Winston-Salem, North Carolina, USA
Buddhika Liyana Pathirannahel: Department of Radiology, Wake Forest School of Medicine, Winston‐Salem, North Carolina, USA
Megan Madonna: Department of Biomedical Engineering, Duke University, Durham, NC, USA
Mark W. Dewhirst: Department of Radiation Oncology, Duke University, Durham, NC, USA
Allen W. Tsang: Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA
Leslie B. Poole: Department of Biochemistry, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA
Nimmi Ramanujam: Department of Biomedical Engineering, Duke University, Durham, NC, USA
S. Bruce King: Department of Chemistry, Wake Forest University, Winston-Salem, North Carolina, USA
Cristina M. Furdui: Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA; Corresponding author.

Journal volume & issue: Vol. 49
p. 102218

Abstract

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Redox metabolism plays essential functions in the pathology of cancer and many other diseases. While several radiotracers for imaging redox metabolism have been developed, there are no reports of radiotracers for in vivo imaging of protein oxidation. Here we take the first step towards this goal and describe the synthesis and kinetic properties of a new positron emission tomography (PET) [18F]Fluoro-DCP radiotracer for in vivo imaging of protein sulfenylation. Time course biodistribution and PET/CT studies using xenograft animal models of Head and Neck Squamous Cell Cancer (HNSCC) demonstrate its capability to distinguish between tumors with radiation sensitive and resistant phenotypes consistent with previous reports of decreased protein sulfenylation in clinical specimens of radiation resistant HNSCC. We envision further development of this technology to aid research efforts towards improving diagnosis of patients with radiation resistant tumors.

Published in Redox Biology

ISSN: 2213-2317 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: http://www.journals.elsevier.com/redox-biology/

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